A 27-year-old man is hospitalized for a 2-day history of fever and headaches and a 1-day history of left lower-extremity weakness. Ten days earlier he had returned from a 1-week trip to Idaho, which involved rafting and sleeping outdoors. He reports no known tick bite.

On physical examination, temperature is 39.1 °C (102.4 °F), blood pressure is 122/72 mm Hg, pulse rate is 118/min, and respiration rate is 28/min. The patient is oriented to person, place, and time. He has mild nuchal rigidity limiting neck flexion. He has left lower-extremity weakness with inability to flex or extend the knee or ankle. Deep tendon reflexes are absent. Sensation is intact. No rash is present.

Cerebrospinal fluid (CSF) studies:

Erythrocyte count

17/µL (17 × 106/L)

Leukocyte count

256/µL (256 × 106/L)

Glucose

55 mg/dL (3.1 mmol/L)

Protein

299 mg/dL (2990 mg/L)

MRI of the brain shows no focal abnormalities.

Which of the following is the most appropriate CSF laboratory test to perform next?